Episode 526 - Capturing biological process in action

Lagrange Point

Seeing how something happens makes it much easier to understand. Biological process can be very hard to capture with images or video. Understanding how a protein requires thinking in 3D but to take images of them we often have to 'snap freeze' them in place. How can lasers, ions and quantum mechanics be used to help capture a protein in motion. PCR based diagnostics tests are accurate but require a lot of setup and expertise. Can you make a PCR test more like a point of care test using bio-luminescence.

1. Shiny Maity, Brad D. Price, C. Blake Wilson, Arnab Mukherjee, Matthieu Starck, David Parker, Maxwell Z. Wilson, Janet E. Lovett, Songi Han, Mark S. Sherwin. Triggered Functional Dynamics of AsLOV2 by Time‐Resolved Electron Paramagnetic Resonance at High Magnetic Fields. Angewandte Chemie International Edition, 2023; 62 (13) DOI: 10.1002/anie.202212832
2. Harmen J. van der Veer, Eva A. van Aalen, Claire M. S. Michielsen, Eva T. L. Hanckmann, Jeroen Deckers, Marcel M. G. J. van Borren, Jacky Flipse, Anne J. M. Loonen, Joost P. H. Schoeber, Maarten Merkx. Glow-in-the-Dark Infectious Disease Diagnostics Using CRISPR-Cas9-Based Split Luciferase Complementation. ACS Central Science, 2023; DOI: 10.1021/acscentsci.2c01467