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DNA on the move – the influence of form on gel migration
Manage episode 463705217 series 3466726
Agarose gel electrophoresis is a staple method in almost all biology and biochemistry lab where separation and analysis of nucleic acids is needed. In this Mol Bio Minutes mini episode Augustė Užuotaitė, Scientist III at Thermo Fisher Scientific, covers the basics of electrophoresis with a spotlight on how different forms of DNA migrate differently in agarose gel electrophoresis.
In less than 10 quick minutes, you’ll learn about the many factors that affect DNA migration rate. Augustė reviews how DNA size, sequence, and conformation all affect migration rate, and she gives some beautifully simple examples to help it all make sense.
Helpful resource links mentioned in this episode:
- Nucleic acid gel electrophoresis – summarized in 5 easy steps
- Five important considerations for the nucleic acid gel electrophoresis
Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.
Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.
For Research Use Only. Not for use in diagnostic procedures.
25 פרקים
Manage episode 463705217 series 3466726
Agarose gel electrophoresis is a staple method in almost all biology and biochemistry lab where separation and analysis of nucleic acids is needed. In this Mol Bio Minutes mini episode Augustė Užuotaitė, Scientist III at Thermo Fisher Scientific, covers the basics of electrophoresis with a spotlight on how different forms of DNA migrate differently in agarose gel electrophoresis.
In less than 10 quick minutes, you’ll learn about the many factors that affect DNA migration rate. Augustė reviews how DNA size, sequence, and conformation all affect migration rate, and she gives some beautifully simple examples to help it all make sense.
Helpful resource links mentioned in this episode:
- Nucleic acid gel electrophoresis – summarized in 5 easy steps
- Five important considerations for the nucleic acid gel electrophoresis
Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.
Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.
For Research Use Only. Not for use in diagnostic procedures.
25 פרקים
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